RESUMO
The sugar mango is a small Colombian cultivar with low fiber content, good aroma and flavor. This research evaluated antioxidant capacity (FRAP, DPPH) of the fruit at different ripening stages classified by visual inspection and CIELab scale. Nutritional and sensory characteristics and ORAC value were measured in the ripe fruit. Sensorial characteristics of two mango nectars sweetened with sucrose and sucralose using ripe fruit were determined by affective acceptance test and Hedonic scale. Results showed that classification by visual inspection coincided with the CIELab scale for all ripening stages. The green mango has 45% more flavonoids than the mature one. The carotenoid content, DPPH and FRAP values were similar in the different ripening stages. Sucralose sweetened nectar was accepted by 94.9% compared with that sweetened with sucrose (89.1%). Results indicate that mango sugar fruit and nectar present antioxidant capacity, bioactive and nutritional compounds with potential health benefits.
El mango de azúcar es una variedad colombiana pequeña, de escasa fibra, buen aroma y sabor. Esta investigación evaluó la capacidad antioxidante del fruto en diferentes estados de maduración por FRAP y DPPH clasificados por inspección visual y escala CIELab. Las características nutricionales y el valor ORAC se determinaron en el mango maduro. Las características sensoriales de dos formulaciones (sacarosa y sucralosa) con mango maduro se determinaron por prueba afectiva de aceptación y escala Hedónica. Resultados: la clasificación por inspección visual coincidió con la escala CIELab. El mango verde tiene 45% más flavonoides que el maduro. El contenido de carotenoides, valores DPPH y FRAP fueron similares en los diferentes estados de maduración. El 94% de los panelistas aceptaron el néctar con sucralosa comparado con el endulzado con sacarosa (89,1%). Los resultados indican que el mango de azúcar y un producto tipo néctar poseen capacidad antioxidante, compuestos bioactivos y nutricionales beneficiosos para la salud.
Assuntos
Mangifera , Percepção Gustatória , Néctar de Plantas , Antioxidantes , Análise de AlimentosRESUMO
The increase of diseases caused by Candida spp., and the treatment failures, has underscored the need for testing the susceptibilities to antifungal agents. The commercial panel ATB® Fungus 2 was compared with the reference testing method of the European Subcommittee on Antifungal Susceptibility Testing of the Committee on Antimicrobial Susceptibility Testing (AFST-EUCAST) for the evaluation of the susceptibility of isolates of Candida spp. to three agents. The percentage of agreement was calculated based on the minimum inhibitory concentrations. There was a high correlation for AMB (100% қ = 1.0 Bhapkar coefficient p = 1.0); while it was lower with azoles (85%, қ = 0.41, p = Bhapkar coefficient 0.02 and 83.0%, қ = 0.15, Bhapkar coefficient p = 0.0006, respectively). The ATB® Fungus 2 and AFST-EUCAST are fully comparable methods for testing the susceptibility to AMB and to lesser extend comparable for ITR and FCA.
El aumento de infecciones por Candida spp. y de las fallas en los tratamientos, suscitan la necesidad de pruebas de susceptibilidad. Se comparó la marca comercial ATB® Fungus 2 con la técnica estándar del Subcomité para las Pruebas de Sensibilidad Antifúngica de la Unión Europea, de la Sociedad de Microbiología Clínica y Enfermedades Infecciosas (AFST-EUCAST) para evaluar la susceptibilidad de aislamientos de Candida spp. a tres antifúngicos. Con base en las concentraciones inhibitorias mínimas se calculó el porcentaje de acuerdo. La concordancia para anfotericina B (AMB) fue alta (100% қ = 1.0, Coeficiente de Bhapkarp = 1.0); para itraconazol (ITR) y fluconazol (FCA) fue inferior (85% қ = 0.41, Coeficiente de Bhapkarp =0.02 y 83.0 %, қ = 0.15, Coeficiente de Bhapkarp = 0.0006, respectivamente). Por lo tanto, ambas técnicas son comparables para la evaluación de la susceptibilidad a AMB; con los azoles el porcentaje de acuerdo es menor.
RESUMO
La porción comestible del mango contiene ácido ascórbico, carotenoides, polifenoles, terpenoides y fibra que tienen efectos protectores para la salud, y posiblemente contra el desarrollo de cáncer de colon (CCO). El objetivo de este estudio fue evaluar la capacidad antiproliferativa en células de adenocarcinoma de colon (SW480) y preventiva en un modelo in vivo de CCO de un extracto acuoso de Mangifera indica cv. Azúcar. El contenido de fenoles totales, flavonoides y carotenoides también fue analizado. El extracto inhibió el crecimiento de las células SW480 en forma dosistiempo- dependiente hasta 22,3% luego de 72h de exposición al extracto (200 μg/mL). La carcinogénesis en el colon de ratones Balb/c fue inducida mediante dos inyecciones intraperitoneales de azoximetano (AOM) a la tercera y cuarta semana de haber iniciado el suministro de mango en el líquido de bebida (0,3%, 0,6%, 1,25%). Después de 10 semanas de tratamiento se observó inhibición dosis-dependiente de la formación de focos de criptas aberrantes (FCA); 0,3% de mango inhibió más del 60% de FCA (p=0,05) comparado con los controles que recibieron agua. Estos resultados muestran que la pulpa del mango de azúcar, un alimento natural, no tóxico, que forma parte de la dieta del ser humano contiene compuestos bioactivos capaces de reducir el crecimiento de células tumorales y prevenir la aparición de las lesiones precancerosas en colon durante el inicio de la carcinogénesis.
Mango pulp contains ascorbic acid, carotenoids, polyphenols, terpenoids and fiber which are healthy and could protect against colon cancer. The aim of this study was to evaluate the antiproliferative and preventive capacity of an aqueous extract of Mangifera indica cv. Azúcar on a human colon adenocarcinoma cell line (SW480) and in a rodent model of colorectal cancer, respectively. The content of total phenolics, flavonoids and carotenoids were also analyzed in the extract. SW480 cell growth was inhibited in a dose and time dependent manner by 22.3% after a 72h exposure to the extract (200 μg/ mL). Colon carcinogenesis was initiated in Balb/c mice by two intra-peritoneal injections of azoxymethane (AOM) at the third and fourth week of giving mango in drinking water (0.3%, 0.6%, 1.25%). After 10 weeks of treatment, in the colon of mice receiving 0.3% mango, aberrant crypt foci formation was inhibited more than 60% (p=0,05) and the inhibition was dose-dependent when compared with controls receiving water. These results show that mango pulp, a natural food, non toxic, part of human being diet, contains bioactive compounds able to reduce growth of tumor cells and to prevent the appearance of precancerous lesions in colon during carcinogenesis initiation.
Assuntos
Animais , Masculino , Anticarcinógenos/farmacologia , Neoplasias do Colo/prevenção & controle , Mangifera/química , Extratos Vegetais/farmacologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Transformação Celular Neoplásica/efeitos dos fármacos , Neoplasias do Colo/induzido quimicamente , Técnicas In Vitro , Camundongos Endogâmicos BALB C , Neoplasias ExperimentaisRESUMO
Mango pulp contains ascorbic acid, carotenoids, polyphenols, terpenoids and fiber which are healthy and could protect against colon cancer. The aim of this study was to evaluate the antiproliferative and preventive capacity of an aqueous extract of Mangifera indica cv. Azúcar on a human colon adenocarcinoma cell line (SW480) and in a rodent model of colorectal cancer, respectively. The content of total phenolics, flavonoids and carotenoids were also analyzed in the extract. SW480 cell growth was inhibited in a dose and time dependent manner by 22.3% after a 72h exposure to the extract (200 µg/ mL). Colon carcinogenesis was initiated in Balb/c mice by two intra-peritoneal injections of azoxymethane (AOM) at the third and fourth week of giving mango in drinking water (0.3%, 0.6%, 1.25%). After 10 weeks of treatment, in the colon of mice receiving 0.3% mango, aberrant crypt foci formation was inhibited more than 60% (p=0,05) and the inhibition was dose-dependent when compared with controls receiving water. These results show that mango pulp, a natural food, non toxic, part of human being diet, contains bioactive compounds able to reduce growth of tumor cells and to prevent the appearance of precancerous lesions in colon during carcinogenesis initiation.
